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. 2005 Aug;16(8):3574–3590. doi: 10.1091/mbc.E05-02-0134

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Figure 5. — The three basic regions within the AKAP79 N-terminal targeting domain mediate polarized localization to MDCK lateral membranes. (A) AKAP79(1-153)-GFP (green) containing all three (A–C) basic regions, (B) AKAP79(1-108)-GFP (green) containing the first two (A and B) basic regions, (C) AKAP79(75-153)-GFP (green) containing the second two (B and C) basic regions and (D) AKAP79(1-ΔB-153)-GFP (green) containing the first and the third (A and C) basic targeting regions all localize to MDCK lateral membranes (xy2 plane) but not to ZO-1 (red) expressing tight junctions (xy1 plane). The transverse plane (xz) shows a lack of colocalization of AKAP constructs (green) with the tight junction marker ZO-1 (red). (E) AKAP79(108-427)-GFP containing a single (C) basic region fails to show membrane localization. (F) Diagram showing primary structures of AKAP79-GFP fragments used in A–E. Bar, 10 μm.