Figure 2.

Expression of genes in the Tetrahymena centrin family. (A) PCR products using primers specific for CEN1, CEN2, CEN3, and CEN4 on cDNA from cells during vegetative growth displayed on an agarose gel. Sizes of select marker (M) bands are displayed in kilobase pairs. (B) Northern blot analysis of RNA from vegetatively growing cells (V), starved cells (S), and conjugating cells at various time points after initiation of mating (2, 4, 6, 9, 18 h) (see Materials and Methods). Similar amounts of total RNA were loaded as assessed by absorbance spectroscopy and ethidium bromide staining our unpublished data).