Figure 5.

Reactivity of the Cen1 antibody. (A) Western immunoblot analysis using the Cen1 antibody as a probe on recombinant 6-histidine–tagged Centrin (lane 1) and Tetrahymena whole cell extract (wce) from cells in logarithmic growth (lane 2). The reactivity of the preimmune serum on wce also is shown (lane 3). (B) Reactivity of the Cen1 antibody with the overexpressed GFP-centrin fusion proteins. Whole cell extracts from strains expressing different fusion proteins were probed with either the Cen1 antibody (left) or a GFP antibody (right). Because the fusion proteins are overexpressed, the signal overwhelms the endogenous Centrin signal, which is visible with longer exposures. Select marker bands are noted and sizes are in kilodaltons.