Figure 6.

Cen1 localization at basal bodies. (A) Immunofluorescence microscopy using the Cen1 antibody and the monoclonal centrin antibody 20H5 raised against Chlamydamonas centrin. DNA is visualized with DAPI. Bar, 10 μm. (B) Serial section immunoelectron microscopy of a basal body in cross section with the Cen1 antibody. Panels proceed from the axoneme distal part of the basal body (a) and track upwards through the structure. Figure 3A serves as a reference for some of the basal body associated structures that are present. Certain basal body associated structures are identified in sections where they are most prominent, although this does not preclude these same structures from being visible in other sections. cw, cartwheel; kf, kinetodesmal fiber; pc, postciliary microtubules; tz, transition zone; tm, transverse microtubules; c, cilia; bb, basal body. Centrin concentrates on the transverse microtubules that are seen obliquely, on the kinetodesmal fiber, and at the transition zone (k). (C) Serial section immunoelectron microscopy of three basal bodies in longitudinal section.