Figure 9.

Cen1 is required for basal body maintenance. (A) Serial sections of a cen1Δ cell (top) and a cell from a wild-type control cross (bottom). The asterisks (*) (top left) indicate the location of one incomplete basal body and a second site of interest. The basal body (top left) does not nucleate a cilium and seems disorganized at its base. The material at the bottom right is unrecognizable as a basal body, although it is present where a basal body would be expected given the location of the mitochondrion on the right (m) and the indentation in the cell surface. In the wild-type panels, three basal bodies (*), two with cilia clearly attached, are visible. Basal bodies are between mitochondria (m), and the cilia emerge from indentations in the cell surface. Bar, 0.5 μm. (B) α-Tubulin localization in cen1Δ cells. Times are after mating initiation of the cen1Δ knockout heterokaryon strains, and cells were stained with a mAb raised against α-tubulin (12G10). By 48 h, many apparent basal bodies do not have cilia attached. Bar, 10 μm.