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. 2005 Aug;16(8):3659–3665. doi: 10.1091/mbc.E05-03-0222

Figure 2.

Figure 2.

Response of HEK293-pmeLUC to extracellular nucleotides (A) and ATP calibration curve (B). HEK293-pmeLUC monolayers were placed in the luminometer chamber as described in Materials and Methods and were then perfused with solutions containing increasing concentrations of nucleotides. Basal cps before addition of the nucleotides ranged between 2500 and 3200. Luminescence increase is shown as percent increase over basal. In B, luminescence increase is correlated to the ATP concentration to build a calibration curve.