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Extracellular ATP triggers release of ATP from P2X7-expressing cells. HEK293 cells transfected with the rat (A) or human (B) P2X7R were placed in the luminometer chamber and perfused with increasing ATP concentrations. HEK293-rP2X7/pmeLUC yellow trace; HEK293-rP2X7/pmeLUC preincubated with 300 μM oATP, blue trace; HEK293-pmeLUC, green trace. HEK293-hP2X7/pmeLUC, red trace; HEK293-hP2X7/pmeLUC preincubated with 300 μM oATP, blue trace; HEK293-pmeLUC, green trace.
