Figure 1.

Clustering of the VC2 region of syndecan-1 cytoplasmic domain is sufficient for cell spreading and fascin-and-actin bundling. (A) Schematic diagram of the chimeric constructs prepared. (B) Equivalent expression of the two proteins in COS-7 cells, as determined by immunoblot with antibody specific for chicken NCAD. Molecular mass markers are indicated in kDa. (C) Morphology and F-actin organization of COS-7 cells expressing either NCADt or NCADt/S1VC2 after plating for 1 h on surfaces coated with 50 μg/ml antibody to chick NCAD extracellular domain and staining with phalloidin. Bar, 10 μm. (D) Quantification of the assay from three independent experiments. Each column represents the mean; bars, SEM. Over 1000 cells were scored in total for each construct.