Table 2.
Surface expression of syndecan-1 proteins in COS7 or IM-9 myeloma cells determined by live-cell staining and flow cytometry
| Sample | % of cells expressing syndecan-1 or heterologous protein | Fluorescent intensity | |
|---|---|---|---|
| A | Isotype control | ND | 11.6 ± 1.3 |
| NCADt | 86.1 ± 1.9 | 263.5 ± 19.5 | |
| NCADt.S1VC2 | 84.5 ± 1.6 | 211.0 ± 25.0 | |
| NCADt.S1V | 84.0 ± 2.0 | 211.0 ± 26.5 | |
| B | |||
| IM9 cells | Isotype control | ND | 22.4 ± 0.9 |
| ID4 (anti-syndecan-1 mab) | 94.4 ± 0.7 | 174.0 ± 7.9 | |
| COS-7 cells | ID4 (anti-syndecan-1 mab) | 87.0 ± 2.0 | 46.0 ± 1.0 |
| C | Isotype control | ND | 29.7 ± 2.3 |
| mS1-WT | 74.0 ± 6.0 | 306.6 ± 28.4 | |
| mS1-T1 | 68.5 ± 13.5 | 353.9 ± 9.2 | |
| mS1-T2 | 73.5 ± 9.5 | 314.0 ± 45.0 | |
| mS1-T3 | 55.5 ± 5.5 | 361.5 ± 28.5 | |
| mS1-T4 | 70.5 ± 9.5 | 370.4 ± 13.4 | |
| D | Isotype control | ND | 35.5 ± 2.4 |
| mSI-WT | 47.0 ± 1.4 | 462.0 ± 74.6 | |
| mS1-K293A | 61.5 ± 1.9 | 620.2 ± 119.0 | |
| mS1-Q294A | 56.1 ± 4.1 | 549.0 ± 119.0 | |
| mS1-N296S/G297V | 51.1 ± 9.2 | 464.8 ± 18.8 | |
| mS1-G297V/G298V | 49.1 ± 0.3 | 586.0 ± 32.0 | |
| mS1-A299F | 56.9 ± 0.9 | 509.0 ± 117.7 | |
| mS1-Y300A | 53.0 ± 5.8 | 345.0 ± 133 | |
| mS1-Y300E | 50.8 ± 1.2 | 561.0 ± 20.0 | |
| mS1-Q301A | 55.0 ± 12.0 | 440.0 ± 136.0 | |
| mS1-K302A | 55.9 ± 3.9 | 471.0 ± 74.0 | |
| mS1-K305A | 56.1 ± 1.6 | 527.0 ± 88.0 | |
| mS1-Q306A | 58.7 ± 4.5 | 611.8 ± 53.4 | |
| mS1-E307A | 53.7 ± 13.7 | 486.7 ± 28.6 | |
| mS1-M4* | 47.2 ± 2.8 | 474.0 ± 63.2 | |
| mS1-M5^ | 59.7 ± 9.2 | 750.9 ± 65.0 | |
| mS1-M5+Y300E | 53.5 ± 3.8 | 609.0 ± 0.1 | |
| mS1-M5+K302A | 56.6 ± 0.5 | 540.0 ± 106.8 | |
| mS1-M5+Q306A | 51.6 ± 1.0 | 497.0 ± 106.0 |
Values are mean ± SEM from 3-5 experiments. ND, not determined.