Figure 1.
Anesthetic and high-temperature response of zzz2 and zzz3(gcn3) mutants. Approximately 5 × 103 cells of the indicated strains were spotted on SC medium and incubated for 3 d at 30°C in the presence of various concentrations of isoflurane (Iso) or in the absence of isoflurane at 37.5°C. ZZZ2 ZZZ3 and GCN3 are alternative names for the RLK88-3C wild-type strain. (A) Growth of the zzz2-1 zzz3-1 double mutant is temperature sensitive and isoflurane resistant. (B) Deletion of GCN3 (gcn3Δ) renders cells resistant to isoflurane.