Table 2.
Plasmids
| Plasmid name | Description | Source |
|---|---|---|
| pL2461 | YCpZZZ3 | This study |
| pL2696 | YCpgcn3::LEU2 | This study |
| p27-1 | YEpglc7Δ209-312 | A. Hinnebusch |
| p180a | YCpGCN4-lacZ with uORFs | A. Hinnebusch |
| p227 | YCpGCN4-lacZ without uORFs | A. Hinnebusch |
| p299 | YCpgcn2-m2 [URA3]b | R. Wek |
| p919 | YCpSUI2 [URA3] | A. Hinnebusch |
| p1097 | YCpSUI2 [LEU2] | A. Hinnebusch |
| p1098 | YCpSUI2-S51A [LEU2] | A. Hinnebusch |
| p1350 | YCpSUI2-L84F [LEU2] | A. Hinnebusch |
| p1751 | YCpgcn20-Δ1 | A. Hinnebusch |
a
Plasmid p180 encodes GCN4-lacZ with the four upstream open reading frames (uORFs) in the 5′ noncoding region of GCN4. Plasmid p227 encodes GCN4-lacZ with mutations destroying the function of all four uORFs of GCN4
b
The gene in the brackets denotes the selectable marker on the plasmid