Table 4.
Effect of drug exposure on GCN4 expression
| β-galactosidase enzyme activitya
|
|||||
|---|---|---|---|---|---|
| 3-AT
|
Isoflurane
|
||||
| Plasmid | uORFsb | - | + | - | + |
| p180 | + | 10.3 ± 0.9 | 49.8 ± 7.6 (4.8)c | 15.6 ± 1.0 | 47.5 ± 1.4 (3.0) |
| p227 | - | 131.1 ± 4.1 | 171.9 ± 5.1 (1.3) | 170.1 ± 5.0 | 222.6 ± 2.7 (1.3) |
a
All values represent the average of at least three independent experiments. Values are reported as the average Miller units ± SEM
b
The significance of the presence or absence of uORFs is described in the text
c
The numbers in parentheses represent the fold-increase in GCN4 expression in drug-treated cells