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. 2005 Aug;16(8):3753–3763. doi: 10.1091/mbc.E05-02-0124

Figure 4.

Figure 4.

Shutoff is delayed in TIA-1-/- MEFs. (A) Total protein production at various times in wt SFV-infected wt and TIA-1-/- MEF cell lines was analyzed as indicated in Materials and Methods. Positions of the cellular protein actin and viral proteins p62, E1, and capsid are indicated. (B) The number of SGs was quantified in wt and TIA-1-/- MEFs infected with SFV-b7EFGP at low MOI. The SGs in 20 cells per time point were counted, and the mean values are plotted. (C) The phosphorylation state of eIF2α in the TIA-1-/- cells 5 h postinfection or mock infection was determined by Western blotting (top) and related to total eIF2α levels by stripping and reprobing the membrane (bottom). (D) vRNA production at the indicated times during infection of wt and TIA-1-/- MEF cell lines was analyzed as indicated in Materials and Methods. Positions of the viral genomic 42S and subgenomic 26S RNAs are indicated. All lanes are labeled in hpi or as M, mock-infected cells.