Figure 5.

SGs in SFV-infected cells are disassembled as vRNA levels increase. (A) ISH analysis of total vRNA at 5 hpi. wt MEFs were infected at low MOI with wt SFV and fixed at 5 hpi. Hybridization and immunostaining was performed as indicated in Materials and Methods. (B) Time course of vRNA accumulation. wt MEFs were infected at low MOI with wt SFV. vRNA produced during the indicated intervals was labeled with BrUTP as indicated in Materials and Methods and processed for immunofluorescence using anti TIA-1 and anti BrUTP. Cells containing SGs are indicated with arrowheads. Bar, 50 μm.