Figure 1.

The FLA8 gene encodes a kinesin-2 motor protein. (A) The FLA8 gene consists of 12 exons separated by 11 introns with the size of each shown. (B) The Fla8 protein is an amino-terminal kinesin predicted to form a 267-amino acid coiled coil domain broken by a 30-amino acid hinge (H). (C) Coomassie Blue-stained SDS-PAGE (7.5% acrylamide) of sucrose density gradient purified kinesin-2 used for MALDI-TOF mass spectrometry analysis. The subunits of 96, 90, and 86 kDa correspond to KAP, Fla10, and Fla8, respectively. TUB, tubulin. (D) Alignment of Fla10 and Fla8 protein sequences. Tryptic peptides identified by mass spectrometry are underlined. Amino acids that are changed in the fla8-1, fla8-2, or fla10-14 alleles are indicated by shading.