Figure 2.

Flagellar loss of fla8 and fla10 mutant alleles when shifted to the restrictive temperature. Compared with wild-type cells (red diamond), the fla10-1 (blue square), fla10-14 (blue circle), fla8-1 (pink square), and fla8-2 (pink triangle) alleles begin to lose their flagella after 3 h at 32°C, whereas fla10-2 (blue triangle) and fla10-14; fla3 (orange triangle) are aflagellate at all temperatures. The fla10-14 intragenic revertants, R16 (green square), R27 (green diamond), R10 (green circle), R8 (green asterisk), and R13 (green triangle) are identical to wild-type cells at the permissive and restrictive temperature.