Figure 5.

Location of amino acids altered in the fla8 and fla10 mutant and revertant alleles as modeled onto the crystal structure of KIF1A with AMPPNP (green) bound (PDB 1VFV). The P-loop is shown in purple. (A and B) Fla10 E₂₄ and L₁₉₆ correspond to KIF1A E₁₉ and M₁₉₆, respectively, and are shown in red. Revertant fla10-14 amino acids K₂₅, N₃₂₉, and G₃₃₁ correspond to KIF1A M₂₀, A₃₃₀, and S₃₃₂, respectively, and are shown in yellow. (C–F) Fla8 amino acids E₂₁ and F₅₅ correspond to KIF1A E₁₉ and F₅₂, respectively, and are shown in red. Revertant fla8-1 and fla8-2 amino acids N₄₂, N₅₇, H₁₀₀, N₃₁₂, M₃₁₃, and G₃₁₄ correspond to KIF1A V₃₈, Y₅₄, Y₁₀₅, A₃₃₀, L₃₃₁, and S₃₃₂, respectively, and are shown in yellow. (G) Alignment of Fla8, Fla10, and KIF1A in the regions of interest. Red shading indicates mutations in one of the kinesin-2 motor subunits, yellow shading indicates amino acids that are altered in the revertants, and purple shading indicates the P-loop residues.