Table 1.

Analysis of intragenic revertants of fla10-14

Strain name	Amino acids of interest	Restriction fragment length polymorphism			Explanation
		E24K25	L196F	G331S
Wild-type	E24K25L196N329	GGAGA	AGGTCCT	GGCCC
	G331	Not cut by MboII	Cut by PpuMI	Cut by Sau96I
fla10-14	E24K; F196	GAAGA	AGGTCTT	GGCCC	Original mutations
		Cut by MboII	Not cut by PpuMI	Cut by Sau96I
R27	K24E; F196	GGAGA	AGGTCTT	GGCCC	True revertant at position 24
		Not cut by MboII	Not cut by PpuMI	Cut by Sau96I
R16	E24 K; K25E; F196	GAAGG	AGGTCTT	GGCCC	Pseudorevertant
		Not cut by MboII	Not cut by PpuMI	Cut by Sau96I
R10,R35 R36, R43	E24K; F196; G331S	GAAGA	AGGTCTT	AGCCC	Pseudorevertant
		Cut by MboII	Not cut by PpuMI	Not cut by Sau96 I
R8	E24K; F196; N329H	GAAGA	AGGTCTT	GGCCC	Pseudorevertant
		Cut by MboII	Not cut by PpuMI	Cut by Sau96I
R13	E24K; F196; N329T	GAAGA	AGGTCTT	GGCCC	Pseudorevertant
		Cut by MboII	Not cut by PpuMI	Cut by Sau96I