Table 1.
Strain name | Amino acids of interest | Restriction fragment length polymorphism | Explanation | ||
---|---|---|---|---|---|
E24K25 | L196F | G331S | |||
Wild-type | E24K25L196N329 | GGAGA | AGGTCCT | GGCCC | |
G331 | Not cut by MboII | Cut by PpuMI | Cut by Sau96I | ||
fla10-14 | E24K; F196 | GAAGA | AGGTCTT | GGCCC | Original mutations |
Cut by MboII | Not cut by PpuMI | Cut by Sau96I | |||
R27 | K24E; F196 | GGAGA | AGGTCTT | GGCCC | True revertant at position 24 |
Not cut by MboII | Not cut by PpuMI | Cut by Sau96I | |||
R16 | E24 K; K25E; F196 | GAAGG | AGGTCTT | GGCCC | Pseudorevertant |
Not cut by MboII | Not cut by PpuMI | Cut by Sau96I | |||
R10,R35 R36, R43 | E24K; F196; G331S | GAAGA | AGGTCTT | AGCCC | Pseudorevertant |
Cut by MboII | Not cut by PpuMI | Not cut by Sau96 I | |||
R8 | E24K; F196; N329H | GAAGA | AGGTCTT | GGCCC | Pseudorevertant |
Cut by MboII | Not cut by PpuMI | Cut by Sau96I | |||
R13 | E24K; F196; N329T | GAAGA | AGGTCTT | GGCCC | Pseudorevertant |
Cut by MboII | Not cut by PpuMI | Cut by Sau96I |