Table 2.
Intragenic revertants of fla8-1 and fla8-2
| Strain | Amino acid change | Codon | Explanation |
|---|---|---|---|
| Wild-type | F55 | ||
| fla8-1 | F55S | TTC to TCC | Original mutation |
| R1, R5, R8, R19, R22, R27 | S55F | TCC to TTC | True revertant |
| R9 | S55Y | TCT to TAT | Pseudorevertant |
| R17 | F55S; N57I | AAT to ATT | Pseudorevertant |
| R29 | F55S; N42H | AAC to CAC | Pseudorevertant |
| R4, R7, R8, R18 | F55S; M313I | ATG to ATA | Pseudorevertant |
| R21, R24 | F55S; M313I; G314S | ATG GGC to ATA AGC | Pseudorevertant |
| fla8-2 | E21K | GAG to AAG | Original mutation |
| R17, R23, R36a | K21E | AAG to GAG | True revertant |
| R10 | E21K; N57Y | AAT to TAT | Pseudorevertant |
| R4, R8d | E21K; H100Y | CAT to TAT | Pseudorevertant |
| R29 | E21K; N312T | AAC to ACC | Pseudorevertant |
| R2, R19, R25, R26 | E21K; M313V | ATG to GTG | Pseudorevertant |
| R8a, R9, R10, R11, R13, R18, R20, R21, R28 | E21K; M313I | ATG to ATA | Pseudorevertant |
| R14, R15, R22, R24, R30, R33, R35 | E21K; M313I | ATG to ATT | Pseudorevertant |
| R16 | E21K; M313I; G314S | ATG GGC to ATA AGC | Pseudorevertant |
| R5, R6, R25, R27, R31, R32, R36b | E21K; G314S | GGC to AGC | Pseudorevertant |