Figure 2.

Representative examples of the subnuclear distribution of H1F0-GFP and H1FOOα-GFP in somatic cells and oocytes. (A and C) Distribution of H1F0-GFP and H1FOOα-GFP in somatic 3134 nuclei. Both H1 isotypes show a distribution characteristic for linker histones. Cells were transiently transfected as described in Materials and Methods. (B and C) Distribution of H1F0-GFP and H1FOOα-GFP in GV stage oocytes. GV-stage oocytes were injected with in vitro-transcribed polyadenylated RNA as previously described (Tanaka et al., 2004). Only H1FOO-GFP shows the stage-specific surrounding nucleolus distribution (B), whereas H1F0-GFP shows aberrant predominantly nucleolar distribution and illuminates only a few nucleoplasmic foci (D). Live cells were monitored using confocal laser scanning microscopy as previously described (Becker et al., 2002). Arrowheads indicate nucleoli; the GV is indicated by a dashed line. Scale bars, 3 mm.