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. 2005 Aug;16(8):3887–3895. doi: 10.1091/mbc.E05-04-0350

Figure 3.

Figure 3.

The globular/N-terminal domain of H1FOO is responsible for the correct subnuclear distribution. (A) Schematic representation of the analyzed H1FOO deletion and chimeric constructs. N, N-terminal domain; G, globular domain; C, C-terminal domain. (B-I) Subnuclear distribution of H1FOO mutants in oocytes (B, D, F, and H) and 3134 cells (C, E, G, and I). (D and E) The N-terminal and globular domain of H1FOO are sufficient to mediated correct localization in oocyte and somatic nuclei. (F and G) The C-terminal domain of H1F0 fused to the N-terminal and globular domain of H1FOO shows a subnuclear localization similar to wild-type H1FOO in oocyte and somatic nuclei. (B, C, H, and I) Neither the C-terminal domain of H1FOO alone (B and C), nor the globular domain of H1F0 fused to the N-terminal and the C-terminal domain of H1FOO (H and I) shows correct localization in oocyte and somatic cell nuclei. Arrowheads indicate nucleoli. Scale bars, 3 mm.