Figure 3.

Defective chromatid cohesion in HeLa cells depleted of EFO1 and/or EFO2. (A) The expression levels of SCC1/MCD1, EFO1, and EFO2 in HeLa cells depleted of EFO1 and/or EFO2. (B) Quantification of the percentage of the unpaired chromosomes in the metaphase spreads prepared from the aforementioned siRNA cells. The standard derivations were calculated based on at least four independent double-blinded experiments and plotted as the error bars. (C) Examples of the metaphase chromosome spreads. The spread of paired sister-chromatids were prepared from mock-transfected HeLa cells, and the spread of unpaired sister-chromatids were prepared from EFO1-siRNA cells. (D) FACS analysis of the siRNA cells described in Figure 2A. (E) Stacks of confocal images of mitotic cells with scattering chromosomes (a and b) and multipole spindles (c and d). The images were taken from double-depleted cells. Chromosomes (blue) were stained with DAPI, and spindles (red) were stained with a monoclonal antibody to α-tubulin.