Figure 7.

Cell cycle regulation of EFO1 and EFO2. (A) Expression levels of EFO1 and EFO2 in cells released from double-thymidine arrest. (B) The expression level and cellular localization of EFO1 and EFO2 in cells released from nocodazole arrest. The time points were indicated above the panels. Phosphatase treatment indicated that the chromosomal EFO1 is specifically phosphorylated during mitosis. Securin and phospho-histone H3 were used as mitotic markers (see text). Topoisomerase IIα (Topo II) and α-actin are used as the marker for the P fractions and the S fractions, respectively.