Figure 1.

The Toprim domain. (A) Alignment of the Toprim domains of RNase M5, DnaG primase, Topoisomerase I and reverse gyrase from different bacteria. BACSU, Bacillus subtilis; BACST, Bacillus stearothermophilus; CLOAC, Clostridium acetobutylicum; STAAU, Staphylococcus aureus; BORBU, Borrelia burgdorferi; AQUAE, Aquifex aeolicus; ECOLI, Escherichia coli; NEIME, Neisseria meningitidis; LACLA, Lactococcus lactis; ARCFU, Archeoglobus fulgidus; METJA, Methanococcus jannaschii; SULSO, Sulfolobus sulfataricus; THEMA, Thermotoga maritima. The colour codes are as follows: turquoise for hydrophobic (A, C, F, I, L, M, V, W, Y), mauve for negatively charged (E, D), green for small (G, S, A), yellow for charged (E, D, R, K, H) and red for positively charged (R, K). Conserved amino acids chosen for mutagenesis are marked with an asterisk. (B) Alignment of the C-terminal domain of RNase M5. Colour codes are the same as in part (A).