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. 2005 Aug 2;33(13):4368–4376. doi: 10.1093/nar/gki752

Figure 3.

Figure 3

5S rRNA maturation by RNase M5 mutants in vivo. (A) Autoradiogram showing a northern blot of total RNA isolated from the different RNase M5 mutants probed for 5S rRNA. The 5S rRNA precursor (pre-5S) and mature 5S rRNA species (5S) are indicated to the left of the autoradiogram. (B) Histogram showing quantification of the gel shown in (A) and those from two further experiments. Individual precursor species were quantified by PhosphorImager and values represent the percent of the total signal present in the 5S rRNA band, normalized to the wild-type (WT) strain, set at 100%.