Figure 6.

Addition of competitive sugar inhibitor blocks lectin expression as determined by immunocytochemistry. Addition of 200 mM galactose can block RCA binding in a hESC colony as shown by lack of RCA binding (6A), but does not affect DAPI stain (blue). However, in the absence of galactose, uniform binding of RCA (red) was detected 6(B). 6A-B) 20× magnification, 100 μm