Table 1.
List of identified xylem sap proteins from Brassica napus. Identifications of xylem sap proteins from the 2-DE gel shown in Figure 2. Sequences were determined from fragmentation spectra obtained by ESI-Q-TOF tandem MS. The resulting partial amino acid sequences were used for database searches with a BLAST algorithm optimized for short sequences. The isobaric amino acids isoleucine (I) and leucine (L), although not distinguishable by MS, are displayed as specified in the corresponding database sequences. BLAST E-values were determined using all non-redundant peptide sequences from one protein spot for another BLAST search. Bold letters in the partial sequences indicate amino acids identical to the database entry. Results of N-terminal secretion sequence predictions are indicated in the right column (Y= secretion sequence predicted, N= no secretion sequence predicted), * indicates that the observed molecular mass was lower and ** indicates that the observed mass was higher than expected.
| Spot No | Similarity to | Organism | Acc. No. | Sequence | identity | E value | expected Mr | observed Mr | aS |
| 1 | Ubiquitin | Arabidopsis thaliana | At2g47110 | TITLEVESSDTIDNVK | 16/16 (100%) | 9.00E-08 | 8525 | 12064 | N |
| 2 | Ubiquitin | Arabidopsis thaliana | At2g47110 | TITLEVESSDTIDNVK | 16/16 (100%) | 9.00E-08 | 8525 | 11944 | N |
| 3 | Glycine-rich cell wall protein | Arabidopsis thaliana | At4g30450 | AGSSAGSFAGSR | 12/12 (100%) | 0.011 | 9530 | 13863 | Y |
| 4 | Pathogenesis-related protein PR1a | Brassica napus | AAB09587 | SPQDYVNAHNQAR | 13/13 (100%) | 7.00E-39 | 17532 | 18145 | Y |
| LAAYAQNYADR | 11/11 (100%) | ||||||||
| QDSPQDYVNAHNQAR | 15/15 (100%) | ||||||||
| GPVQWDGTLAAYAQNYADR | 19/19 (100%) | ||||||||
| AGSSADFSGVSAVNLWVNEK | 20/20 (100%) | ||||||||
| 5 | Glycine-rich cell wall protein | Oryza sativa | BAD62284 | GGGYGEGGGYGGG | 15/16 (94%) | 2.00E-10 | 14152 | 17822 | Y |
| GGGYWGGGYGGGYGGG | |||||||||
| 6 | Invertase/pectin methylesterase inhibitor | Arabidopsis thaliana | At1 g47960 | GSSADTSGLALILVDK | 15/16 (94%) | 2.00E-32 | 22326 | 18297 | Y |
| GVIDAGVEAAV | 10/11 (91%) | ||||||||
| ETPDFNLCVACPLDSDPR | 15/18 (83%) | ||||||||
| GSSADTSGLALILVDKIK | 17/18 (94%) | ||||||||
| ADVPEAIECCSK | 10/12 (83%) | ||||||||
| FGEDGVIDAGVEAAV | 14/15 (93%) | ||||||||
| 7 | Papain-type cysteine proteinase XCP2 | Arabidopsis thaliana | At4g35350 | LDHGVAAVGYGSSK | 14/14 (100%) | 4.00E-18 | 39618 | 16,603* | Y |
| ALAHQPVSVAIEASGR | 16/16 (100%) | ||||||||
| GVDLDHGVAAVGYGSSK | 16/17 (94%) | ||||||||
| 8 | Papain-type cysteine proteinase XCP2 | Arabidopsis thaliana | At4g35350 | LDHGVAAVGYGSSK | 14/14 (100%) | 4.00E-18 | 39618 | 16,977* | Y |
| ALAHQPVSVAIEASGR | 16/16 (100%) | ||||||||
| GVDLDHGVAAVGYGSSK | 16/17 (94%) | ||||||||
| 9 | Invertase/pectin methylesterase inhibitor | Arabidopsis thaliana | At1g47960 | GSSADTSGLALILVDK | 15/16 (94%) | 9.00E-38 | 22326 | 18297 | Y |
| ETPDFNLCVACPLDSDPR | 15/18 (83%) | ||||||||
| GSSADTSGLALILVDKIK | 17/18 (94%) | ||||||||
| ALDECASR | 6/8 (75%) | ||||||||
| ADVPEAIECCSK | 10/12 (83%) | ||||||||
| FGEDGVIDAGVEAAV | 14/15 (93%) | ||||||||
| 10 | Glycine-rich protein | Arabidopsis thaliana | At4g30460 | GEGGGYGGGYGGGGD | 14/15 (93%) | 1.00E-04 | 13738 | 19195 | Y |
| 11 | Aspartyl protease family protein | Arabidopsis thaliana | At5g07030 | LPPSAIAFNPATGAGTIFDSGTVYTR | 24/26 (92%) | 3.00E-15 | 46919 | 21,384* | Y |
| VLIDLPNSR | 7/9 (77%) | ||||||||
| 12 | Disease resistance response/ dirigent protein | Arabidopsis thaliana | At1g64160 | VIFDDPVTLDK | 8/12 (67%) | 3.00E-13 | 20573 | 22711 | Y |
| GCLNIMGADL | 9/10 (90%) | ||||||||
| DLSVVGGTGDFFFSR | 14/15 (93%) | ||||||||
| 13 | Disease resistance response/ dirigent protein | Arabidopsis thaliana | At4g23690 | VIFDDPVTLDQNYLSKPVSR | 17/20 (85%) | 1.00E-11 | 21412 | 24544 | Y |
| SVVGGTGDFFMSR | 13/13 (100%) | ||||||||
| 14 | Endochitinase class I, putative | Arabidopsis thaliana | At2g43620 | RDTIANVVTLSVFNSIFSK | 12/19 (63%) | 2.00E-08 | 30378 | 25856 | Y |
| QAFISAAAQSSDAYK | 13/15 (87%) | ||||||||
| FNGLPLLTDPDMVSR | 13/15 (87%) | ||||||||
| 15 | Endochitinase class I, putative | Arabidopsis thaliana | At2g43620 | RDTIANVVTLSVFNSIFSK | 12/19 (63%) | 2.00E-08 | 30378 | 25969 | Y |
| QAFISAAAQSSDAYK | 13/15 (87%) | ||||||||
| FNGLPLLTDPDMVSR | 13/15 (87%) | ||||||||
| 16 | Endochitinase class I, putative | Arabidopsis thaliana | At2g43620 | RDTIANVVTLSVFNSIFSK | 12/19 (63%) | 7.00E-13 | 30378 | 25744 | Y |
| QAFISAAAQSSDAYK | 13/15 (87%) | ||||||||
| FNGLPLLTDPDMVSR | 13/15 (87%) | ||||||||
| PVLDQGFGATTR | 12/12 (100%) | ||||||||
| 17 | Germin-like protein, subfamily 2 | Arabidopsis thaliana | At1 g02335 | YDPDALQDLCVAADK | 14/15 (93%) | 8.00E-07 | 23441 | 26543 | Y |
| KIPGLNTLSV | 10/10 (100%) | ||||||||
| GEVFVFPR | 8/8 (100%) | ||||||||
| 18 | Thaumatin-like protein/ osmotin | Brassica rapa | AAN23104 | RAPNTLAEYALK | 12/12 (100%) | 4.00E-04 | incomplete | 32060 | |
| 19 | Gamma-glutamyltranspeptidase 1 | Arabidopsis thaliana | At4g39640 | PPPAPANFIRPGK | 13/13 (100%) | 4.00E-D5 | 61190 | 26,312* | Y |
| VPPPAPANFIRPGK | 14/14 (100%) | ||||||||
| QFIVQES | 7/7 (100%) | ||||||||
| 20 | Endochitinase class I, putative | Arabidopsis thaliana | At2g43620 | TIANVVTLSVFNSIFSK | 10/17 (59%) | 2.00E-20 | 30378 | 28613 | Y |
| QAFISAAAQSSDAYK | 13/15 (87%) | ||||||||
| FLGLPLLTDPDFVAR | 12/15 (80%) | ||||||||
| IQITSDNYNYGAAGK | 12/17 (71%) | ||||||||
| 21 | Xyloglucan:xyloglucosyl transferase | Arabidopsis thaliana | At5g57560 | GQITNDGELLTLSLDK | 14/16 (88%) | 3.00E-36 | 32093 | 29928 | Y |
| LVPGNSAGTVTTLYLK | 16/16 (100%) | ||||||||
| WFDPTWFHTYTILSDNPQR | 16/19 (84%) | ||||||||
| IFTVDGTPIR | 10/10 (100%) | ||||||||
| NFESVGTLFPNNKPFR | 13/16 (81%) | ||||||||
| 22 | Xyloglucan:xyloglucosyl transferase | Arabidopsis thaliana | At5g57560 | GQITNDGELLTLSLDK | 14/16 (88%) | 3.00E-36 | 32093 | 30200 | Y |
| LVPGNSAGTVTTLYLK | 16/16 (100%) | ||||||||
| WFDPTVVFHTYTILSDNPQR | 16/19 (84%) | ||||||||
| IFTVDGTPIR | 10/10 (100%) | ||||||||
| NFESVGTLFPNNKPFR | 13/16 (81%) | ||||||||
| 23 | Chitinase class IV, putative | Arabidopsis thaliana | At2g43570 | PSFGSSISK | 8/9 (89%) | 2.00E-05 | 29775 | 31326 | Y |
| AAANSYPSFGSSISK | 14/15 (93%) | ||||||||
| LNENLLASPEK | 10/11 (91%) | ||||||||
| VAQDPPSAFK | 7/10 (70%) | ||||||||
| 24 | Peroxidase 25 precursor | Arabidopsis thaliana | At2g41480 | STVESHFDSDPTISPGLLR | 19/19 (100%) | 4.00E-09 | 35886 | 32665 | Y |
| EASNLPSPLDSVAVQK | 16/16 (100%) | ||||||||
| VALDIGSPSNFDVSFFK | 15/17 (88%) | ||||||||
| 25 | Legume lectin family protein | Arabidopsis thaliana | At3g16530 | LAVEFDTFQNK | 10/11 (91%) | 4.00E-06 | 30509 | 32360 | Y |
| GYWVQTR | 7/7 (100%) | ||||||||
| Spot No | Similarity to | Organism | Acc. No. | Sequence | identity | E value | expected Mr | observed Mr | aS |
| 26 | Legume lectin family protein | Arabidopsis thaliana | At3g15356 | GDNLFFLGDAELG | 11/13(85%) | 7.00E-15 | 29749 | 32819 | Y |
| EFDTFKNK | 7/8 (88%) | ||||||||
| HVGININSMTSNVAEK | 15/16 (94%) | ||||||||
| TITIAPENVK | 10/10 (100%) | ||||||||
| 27 | Chitinase class I | Brassica napus | CAA43708 | DSFINAANTFPNFANSVTR | 19/19 (100%) | 5.00E-11 | 28733 | 30200 | Y |
| NGGNSGAVNAR | 11/11 (100%) | ||||||||
| 28 | Legume lectin family protein | Arabidopsis thaliana | At3g16530 | LAVEFDTFQNK | 9/10 (90%) | 5.00E-06 | 30509 | 31763 | Y |
| AGYWVQTR | 8/8 (100%) | ||||||||
| 29 | Chitinase class I, putative | Arabidopsis thaliana | At4g01700 | GFYPYEAFVEATR | 13/13 (100%) | 1.00E-08 | 31464 | 32819 | Y |
| GPIQLSWNYNYGQAGR | 16/16 (100%) | ||||||||
| 30 | Peroxidase, putative | Arabidopsis thaliana | At4g36430 | PGTVSCAADLLTLAAR | 14/16 (88%) | 4.00E-05 | 36164 | 34420 | Y |
| QNLSVLDIVSAAK | 10/13 (77%) | ||||||||
| GNISPLTGSSGEIR | 14/14 (100%) | ||||||||
| 31 | Peroxidase, putative | Arabidopsis thaliana | At4g36430 | DSSVLTGGPCWVVP | 13/14 (93%) | 7.00E-18 | 36164 | 36508 | Y |
| QGLDITDLVALSGSHTIGFSR | 21/21 (100%) | ||||||||
| NSDQVLFSS | 9/9 (100%) | ||||||||
| GNISPLTGSSGEIR | 14/14 (100%) | ||||||||
| 32 | Peroxidase, putative | Arabidopsis thaliana | At1g49570 | EAVVLTGGPFMAPVPLGR | 16/18 (89%) | 9.00E-06 | 38030 | 41164 | Y |
| GQPDPNLAASSDLLSK | 15/16 (94%) | ||||||||
| GNIGVFTGSDGVIR | 13/14 (93%) | ||||||||
| 33 | Peroxidase ATP5a | Arabidopsis thaliana | At1g49570 | EAVVLTGGPFMAPVPLGR | 16/18 (89%) | 7.00E-05 | 38030 | 40065 | Y |
| GNIGVFTGSDGVIR | 13/14 (93%) | ||||||||
| Peroxidase 59 precursor | Arabidopsis thaliana | At5g19890 | LVEAYSQSQSLFFR | 13/14 (93%) | 6.00E-05 | 35023 | 40065 | Y | |
| 34 | Serine carboxypeptidase S10 family protein | Arabidopsis thaliana | At3g17180 | GFIVGNPLTDDEYDNK | 16/16 (100%) | 1.00E-08 | 54145 | 40,498* | Y |
| 35 | Curculin-like (mannose-binding) lectin | Arabidopsis thaliana | At1g78830 | PLVYGGDSPNHDFR | 11/14 (79%) | 2.00E-09 | 50343 | 39,640 | Y |
| VVNNDEFGDYITEYDASYR | 16/19 (84%) | ||||||||
| GVTGFEILPNGNIVLHDK | 17/18 (94%) | ||||||||
| TGQPLVYGGDSPNHDFR | 13/17 (76%) | ||||||||
| 36 | Curculin-like (mannose-binding) lectin | Arabidopsis thaliana | At1g78830 | PLVYGGDSPNHDFR | 11/14 (79%) | 2.00E-09 | 50343 | 36,326* | Y |
| VVNNDEFGDYITEYDASYR | 16/19 (84%) | ||||||||
| GVTGFEILPNGNIVLHDK | 17/18 (94%) | ||||||||
| TGQPLVYGGDSPNHDFR | 13/17 (76%) | ||||||||
| 37 | Peroxidase, putative | Arabidopsis thaliana | At4g33420 | PTLSAGLIR | 8/9 (89%) | 1.00E-17 | 35975 | 37062 | Y |
| NDFDNAYFNALQR | 13/13 (100%) | ||||||||
| SGVLFSDQTLFNSPFTR | 15/17 (88%) | ||||||||
| 38 | Curculin-like (mannose-binding) lectin | Arabidopsis thaliana | At1g78830 | PLVYGGDSPNHDFR | 11/14 (79%) | 3.00E-08 | 50343 | 37,631* | Y |
| VVNNDEFGDYITEYDASYR | 16/19 (84%) | ||||||||
| ILPNGNIVLHDK | 12/1 (100%) | ||||||||
| TGQPLVYGGDSPNHDFR | 13/17 (76%) | ||||||||
| 39 | Curculin-like (mannose-binding) lectin | Arabidopsis thaliana | At1g78830 | PLVYGGDSPNHDFR | 11/14 (79%) | 3.00E-08 | 50343 | 35,967* | Y |
| VVNNDEFGDYITEYDASYR | 16/19 (84%) | ||||||||
| ILPNGNIVLHDK | 12/1 (100%) | ||||||||
| TGQPLVYGGDSPNHDFR | 13/17 (76%) | ||||||||
| 40 | Curculin-like (mannose-binding) lectin | Arabidopsis thaliana | At1g78830 | PLVYGGDSPNHDFR | 11/14 (79%) | 3.00E-08 | 50343 | 37,824* | Y |
| VVNNDEFGDYITEYDASYR | 16/19 (84%) | ||||||||
| ILPNGNIVLHDK | 12/1 (100%) | ||||||||
| TGQPLVYGGDSPNHDFR | 13/17 (76%) | ||||||||
| 41 | Curculin-like (mannose-binding) lectin | Arabidopsis thaliana | At1g78830 | PLVYGGDSPNHDFR | 11/14 (79%) | 3.00E-08 | 50343 | 36,326* | Y |
| VVNNDEFGDYITEYDASYR | 16/19 (84%) | ||||||||
| ILPNGNIVLHDK | 12/1 (100%) | ||||||||
| TGQPLVYGGDSPNHDFR | 13/17 (76%) | ||||||||
| 42 | Peroxidase 59 precursor | Arabidopsis thaliana | At5g19890 | KNAIPNINSAR | 10/11 (91%) | 0.003 | 35023 | 40280 | Y |
| EAYSQSQSLFFR | 12/12 (100%) | ||||||||
| 43 | Peroxidase, putative | Arabidopsis thaliana | At4g33420 | PTLAAGLIR | 9/9 (100%) | 1.00E-09 | 35975 | 37824 | Y |
| NTAEKDSPANLSLR | 14/14 (100%) | ||||||||
| NDFDNAYFNALQR | 13/13 (100%) | ||||||||
| 44 | Cyclase family protein | Arabidopsis thaliana | At4g34180 | DYLSFAAFDESPATHK | 15/16 (94%) | 1.00E-28 | 28384 | 34586 | Y |
| GLDYLSFAAFDESPATHK | 17/18 (94%) | ||||||||
| EFDSSFSGFFTDGAK | 13/15 (87%) | ||||||||
| LIGLDYLSFAAFDESPATHK | 19/20 (95%) | ||||||||
| GRDIIPVEALK | 11/11 (100%) | ||||||||
| 45 | Peroxidase 21 (ATP2a) | Arabidopsis thaliana | At2g37130 | DASLLLETAR | 10/10 (100%) | 5.00E-18 | 36741 | 35614 | Y |
| PTLDPDYALYLK | 11/12 (92%) | ||||||||
| ADNGYFHEQFSR | 12/12 (100%) | ||||||||
| LLSETNPLTGDQGEIR | 16/16 (100%) | ||||||||
| 46 | Peroxidase, putative | Arabidopsis thaliana | At4g33420 | PTLAAGLIR | 9/9 (100%) | 8.00E-40 | 35975 | 38018 | Y |
| GFTPQDVVALSGAHTLGVAR | 20/20 (100%) | ||||||||
| LTTPDSSFDSSFVNTLTK | 13/18 (72%) | ||||||||
| FDNAYFNALQR | 10/11 (91%) | ||||||||
| SGVLFSDQTLFNTPATR | 17/17 (100%) | ||||||||
| 47 | Peroxidase, putative | Arabidopsis thaliana | At4g33420 | DNTAEKDSPANLSLR | 15/15 (100%) | 1.00E-25 | 35975 | 37062 | Y |
| GFTPQDVVALSGAHTLGVAR | 20/20 (100%) | ||||||||
| NDFDNAYFNALQR | 12/13 (92%) | ||||||||
| LFSDQTLFNTP | 11/11 (100%) | ||||||||
| 48 | Peroxidase 21 (ATP2a) | Arabidopsis thaliana | At2g37130 | DASLLLETAR | 10/10 (100%) | 8.00E-09 | 36741 | 36691 | Y |
| LLSETNPLTGDQGEIR | 16/16 (100%) | ||||||||
| 49 | Beta-1,3-glucanase (BG3) | Arabidopsis thaliana | At3g57240 | LLLDVPNPDLQR | 11/12 (92%) | 3.00E-1B | 30712 | 36691 | N |
| YISVGNEVQPS | 11/11 (100%) | ||||||||
| FVLPAMQNIDR | 10/11 (91%) | ||||||||
| TYVNNLIQTVK | 11/11 (100%) | ||||||||
| Spot No | Similarity to | Organism | Acc. No. | Sequence | identity | E value | expected Mr | observed Mr | aS |
| 50 | Beta-1,3-glucanase (BG3) | Arabidopsis thaliana | At3g57240 | LLLDVPNPDLQR | 11/12(92%) | 3.00E-18 | 30712 | 37250 | N |
| YISVGNEVQPS | 11/11 (100%) | ||||||||
| FVLPAMQNIDR | 10/11 (91%) | ||||||||
| TYVNNLIQTVK | 11/11 (100%) | ||||||||
| 51 | Polygalacturonase inhibitor protein 2 | Brassica napus | AAM94869 | NFLQFDLSR | 9/9 (100%) | 5.00E-05 | 37117 | 40280 | Y |
| LQEFDTYSYFHNK | 13/13 (100%) | ||||||||
| 52 | Peroxidase 59 precursor | Arabidopsis thaliana | At5g19890 | PYLVQIVR | 7/8 (88%) | 2.00E-06 | 35023 | 41850 | Y |
| GFEVVDTIK | 8/9 (89%) | ||||||||
| LVEAYSQSQSLFFR | 13/14 (93%) | ||||||||
| 53 | Peroxidase 59 precursor | Arabidopsis thaliana | At5g19890 | PYLVQIVR | 7/8 (88%) | 5.00E-04 | 35023 | 43793 | Y |
| LVEAYSQSQSLFFR | 13/14 (93%) | ||||||||
| 54 | Peroxidase 59 precursor | Arabidopsis thaliana | At5g19890 | PYLVQIVR | 7/8 (88%) | 2.00E-06 | 35023 | 43793 | Y |
| GFEVVDTIK | 8/9 (89%) | ||||||||
| LVEAYSQSQSLFFR | 13/14 (93%) | ||||||||
| 55 | Subtilase family protein | Arabidopsis thaliana | At1g20160 | AVASAYGTFPTTVIDSK | 16/17 (94%) | 1.00E-17 | 81480 | 56,025* | Y |
| SILKPDITAPGVAILAAWTG | 19/20 (95%) | ||||||||
| LTYQVTVSATA | 10/11 (91%) | ||||||||
| 56 | Subtilase family protein | Arabidopsis thaliana | At1g20160 | SPVYPLIHGK | 10/10 (100%) | 2.00E-05 | 81480 | 56,025* | Y |
| ASAYGTFPTTVIDSK | 14/15 (93%) | ||||||||
| VSVETPAYQIQVTPEK | 13/16 (63%) | ||||||||
| VFGALT | 6/6 (100%) | ||||||||
| 57 | Curculin-like (mannose-binding) lectin | Arabidopsis thaliana | At1g78850 | KVPVDEQFLVVNK | 10/13 (77%) | 8.00E-16 | 49051 | 50573 | Y |
| TDYSPIEYNPDVR | 13/13 (100%) | ||||||||
| ILENGNFVIYDSSVK | 13/15 (87%) | ||||||||
| SVNTDGPYSLVFEDK | 12/15 (80%) | ||||||||
| TPKPIAVREGYEFFSK | 12/16 (75%) | ||||||||
| 58 | Polygalacturonase/ glycoside hydrolase 28 | Arabidopsis thaliana | At3g61490 | DATLLAAQDLEEYPVLK | 14/17 (82%) | 1.00E-10 | 51939 | 56471 | Y |
| DEYGIAFGFPTK | 10/12 (83%) | ||||||||
| IALGSEFSGGIEDVR | 14/15 (93%) | ||||||||
| 59 | Subtilase family protein | Arabidopsis thaliana | At1g20160 | SPVYPLIHGK | 10/10 (100%) | 1.00E-10 | 81480 | 58,848* | Y |
| ASAYGTFPTTVIDSK | 14/15 (93%) | ||||||||
| SLHPTWCPSAIR | 10/12 (83%) | ||||||||
| VSVETPAYQIQVTPEK | 13/16(81%) | ||||||||
| LTYQVTVSAADDDV | 10/14(71%) | ||||||||
| VFGALT | 6/6 (100%) | ||||||||
| 60 | Subtilase family protein | Arabidopsis thaliana | At1g20160 | SPVYPLIHGK | 10/10 (100%) | 1.00E-10 | 81480 | 56,025* | Y |
| ASAYGTFPTTVIDSK | 14/15 (93%) | ||||||||
| SLHPTWCPSAIR | 10/12 (83%) | ||||||||
| VSVETPAYQIQVTPEK | 13/16(81%) | ||||||||
| LTYQVTVSAADDDV | 10/14(71%) | ||||||||
| VFGALT | 6/6 (100%) | ||||||||
| 61 | Curculin-like (mannose-binding) lectin | Arabidopsis thaliana | At1g78850 | KVPVDEQFLVVNK | 10/13 (77%) | 6.00E-11 | 49051 | 50573 | Y |
| TDYSPIEYNPDVR | 13/13 (100%) | ||||||||
| ILENGNFVIYDSSVK | 13/15 (87%) | ||||||||
| SVNTDGPYSLVFEDK | 12/15 (80%) | ||||||||
| TPKPIAVREGYEFFSK | 12/16 (75%) | ||||||||
| 62 | Curculin-like (mannose-binding) lectin | Arabidopsis thaliana | At1g78850 | KVPVDEQFLVVNK | 10/13 (77%) | 6.00E-11 | 49051 | 51999 | Y |
| TDYSPIEYNPDVR | 13/13 (100%) | ||||||||
| ILENGNFVIYDSSVK | 13/15 (87%) | ||||||||
| SVNTDGPYSLVFEDK | 12/15 (80%) | ||||||||
| TPKPIAVREGYEFFSK | 12/16 (75%) | ||||||||
| 63 | Subtilase family protein | Arabidopsis thaliana | At1g20160 | AVASAYGTFPTTVIDSK | 16/17 (94%) | 3.00E-07 | 81480 | 56,926* | Y |
| TVTNVGGDRAVYK | 10/13 (77%) | ||||||||
| VFGALT | 6/6 (100%) | ||||||||
| 64 | Polygalacturonase/ glycoside hydrolase 28 | Arabidopsis thaliana | At3g61490 | DEYGIAFGFPTK | 10/12 (83%) | 9.00E-08 | 51939 | 5786B | Y |
| IALGSEFSGGIEDVR | 14/15 (93%) | ||||||||
| WTLLAAQDLEEYPVLK | 14/16 (88%) | ||||||||
| 65 | Subtilase family protein | Arabidopsis thaliana | At1g20160 | ESDVVLGGSK | 9/10 (90%) | 5.00E-08 | 81480 | 81188 | Y |
| SPVYPLIHGK | 10/10 (100%) | ||||||||
| AVASAYGTFPTTVIDSK | 16/17 (94%) | ||||||||
| SLHPTWCPSAIR | 10/12 (83%) | ||||||||
| TVTNVGGDRAVYK | 10/13 (77%) | ||||||||
| LTYQVTVSA | 8/9 (89%) | ||||||||
| 66 | Subtilase family protein | Arabidopsis thaliana | At1g20160 | ESDVVLGGSK | 9/10 (90%) | 5.00E-08 | 81480 | 79849 | Y |
| SPVYPLIHGK | 10/10 (100%) | ||||||||
| AVASAYGTFPTTVIDSK | 16/17 (94%) | ||||||||
| SLHPTWCPSAIR | 10/12 (83%) | ||||||||
| TVTNVGGDRAVYK | 10/13 (77%) | ||||||||
| LTYQVTVSA | 8/9 (89%) | ||||||||
| 67 | Subtilase family protein | Arabidopsis thaliana | At1g20160 | ESDVVLGGSK | 9/10 (90%) | 6.00E-07 | 81480 | 81188 | Y |
| AVASAYGTFPTTVIDSK | 16/17 (94%) | ||||||||
| TVTNVGGDRAVYK | 10/13 (77%) | ||||||||
| LTYQVTVSA | 8/9 (89%) | ||||||||
| VFGALT | 6/6 (100%) | ||||||||
| 68 | Cucumisin-like serine protease (ARA12) | Arabidopsis thaliana | At5g67360 | VEGASLLGFASGTAR | 14/15 (93%) | 1.00E-06 | 79415 | 78587 | Y |
| DGVAIGAFAAFER | 12/13 (92%) | ||||||||
| PALAILGNGK | 10/10 (100%) | ||||||||
| LNYPSFAVNVDGSGAYK | 16/17 (94%) | ||||||||
| TVTFTVDSSK | 10/10 (100%) | ||||||||
| 69 | Germin-like protein | Arabidopsis thaliana | At3g05950 | FTSGLNIAGNTIN | 13/13 (100%) | 7.00E-05 | 24736 | 76,260** | Y |
| AFQLDVNVVR | 9/10 (90%) | ||||||||