Table 1.

Overview of various applications of the NICE system

Application area	Application	Reference
Expression of homologous genes	Aminopeptidase N	[7]
	ATPase system (8 gene operon)	[28]
	Aminoacylase	[29]
	Cluster of genes encoding folate biosynthesis	[19]
Expression of heterologous genes of Gram+ and Gram- bacteria	NADH oxidase of Streptococcus mutans	[30]
	Fructose bisphosphatase of Escherichia coli	[31]
	Green fluorescent protein of Aequoria victoria	[32]
	Lysostaphin of Staphylococcus simulans biovar. Staphylolyticus	[15]
Protein secretion	Lipase of Staphylococcus hyicus	[33]
	Bovine beta-lactoglobulin	[34]
Membrane proteins: prokaryotic and eukaryotic	Multidrug transporter of Lactococcus lactis
	Xylidose transporter of Lactobacillus pentosus
	ATP/ADP translocator of Rickettsia prowazekii	Review [35]
	KDEL receptor of Homo sapiens
	Mitochondrial carriers of Saccharomyces cerevisiae
Cloning of toxic genes	Lysis cassette of the virulent phage us3 of	[26]
	Lactococcus lactis
	Autolysin gene of Leuconostoc citreum	[36]
Bacterial antigens	Antigen L7/12 of Brucella abortus	[13, 37]
	C subunit of tetanus toxin (TTFC) of Clostridium tetani	[38]
Viral antigens	Non-structural protein 4 of bovine rotavirus	[13, 39]
Cytokines	Interleukin 12 of Homo sapiens	[40]
Industrial-scale application	Lysostaphin of Staphylococcus simulans biovar. Staphylolyticus	[15]