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. 2005 Jul 22;102(31):10887–10892. doi: 10.1073/pnas.0409283102

Fig. 5.

Fig. 5.

Isolation of CFTRΔF508 aggregates under nondenaturing conditions. (a) Constructs. GFP tag is indicated by black box, and affinity tags (protein A and CBP) for isolation of CFTRΔF508 aggregates are indicated by gray boxes. TEV, TEV protease recognition sequence. (b) Immunoblotting of column fractions was performed by using anti-GFP antibody (JL-8). The individual fractions are designated by the convention introduced in Fig. 1b. Molecular mass markers (in kDa) are indicated on the left. (c and d) TEM of uranyl acetate-stained immunogold-labeled CFTRΔF508 aggregates. The size of the gold particles was 10 nm. (Scale bar, 100 nm.) (e–h) The AFM height images of CFTRΔF508 aggregates (e and f) and the surface profiles (g and h) along the A–B (e and g) and C–D (f and h) axes, respectively. The arrows in e indicate the globular aggregates, corresponding to the arrows in g. The arrows in f indicate the globular aggregates, corresponding to the arrows in h.