Fig. 2.

Role of PI (3)/Akt kinase pathway in DHA's antiapototic effect. (A) The presence of wortmanin (WM) (10 nM) or LY 294002 (LY) (50 μM) during serum starvation abolished DHA's inhibition of caspase-3 activity in serum-starved cells. Results are percentages of the nonenriched control value, and statistical significance was tested against serum-free nonenriched control. ***, P < 0.001. (B) Western blot data showing the reduction of Akt phosphorylation upon serum starvation and the prevention of this reduction by DHA and abolition of DHA's protection by WM and LY. (C) Akt activity maintained by DHA during serum deprivation. Results are representative of at least two independent experiments.