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. 2005 Jul 22;102(31):10898–10903. doi: 10.1073/pnas.0504834102

Fig. 3.

Fig. 3.

Loss of Dicer in the mouse limb leads to cell death. A, C, and E show wild-type limbs, and B, D, and F show limbs in which Dicer has been removed from the limb mesoderm using the prx1cre allele. An increase in cell death in Dicer-minus limbs compared with age-matched littermates was observed in E10.5 limbs (compare A and B). By E11.5, Dicer-minus limbs were narrower than wild-type limbs and exhibited an increase in cell death throughout the limb mesoderm. Note that loss of a single copy of Dicer does not lead to an increase in cell death (compare C and D). By E12.5, a wild-type pattern of cell death was observed in the apical ectodermal ridge (AER) of wild-type limbs (arrow in E). Dicer-minus limbs exhibited cell death in the AER in addition to ectopic cell death throughout the limb mesoderm (F). Dicer-minus limbs were also smaller and digit condensations were not visible in E12.5 mutant limbs. The ×2 magnification insets in E and F are of the distal, posterior region of the respective limbs. All pictures are of forelimbs from littermates. Images in A–D were taken at the same magnification.