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. 2005 Aug;17(8):2157–2171. doi: 10.1105/tpc.105.033449

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Copyright © 2005, American Society of Plant Biologists

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Figure 10. — Phenotypes of 35S:MIR165a Transgenic Plants.

(A) A 35S:MIR165a/Ler seedling, showing small and round leaves with margins slightly curled downward. C, cotyledon.

(B) A 35S:MIR165a/as2-101 seedling with ruffled leaf surfaces. Arrowheads indicate the lotus leaves.

(C) A 35S:MIR165a/rdr6-3 seedling. First-pair rosette leaves resemble those of the as2-101 mutant plants.

(D) A 35S:MIR165a/rdr6-3 transgenic plant showing a lotus leaf structure (arrowhead).

(E) A 35S:MIR165a/rdr6-3 transgenic plant displaying severe phenotypes with abaxialized needle-like leaves (arrowheads).

(F) Comparison of venation between wild-type (left) and 35S:MIR165a/Ler (right) leaves, showing that venation of the 35S:MIR165a/Ler transgenic line was simplified. Bars = 1 mm in (A) to (F).

(G) Quantitative analyses of NBPs in wild-type and 35S:MIR165a/Ler transgenic plants.

(H) Real-time RT-PCR to examine steady state levels of mRNA of class III HD-ZIP genes in wild-type and 35S:MIR165a/Ler plants. Bars show standard error.