Figure 9.

Expression of the FIL Gene.

(A) RT-PCR detection of FIL transcript levels in wild-type, as2-101, rdr6-3, and rdr6-3 as2-101 leaves. Numbers indicate the relative abundance of gene transcripts and were calculated using band intensity of the first lane (Ler) as 1.0.

(B) and (C) In situ hybridization using an antisense FIL probe to transverse sections of leaf primordia in wild-type Ler (B) and rdr6-3 as2-101 (C) that is in the Ler genetic background. Note that the FIL expression was extended to the entire leaf primordium (arrow) and the adaxial side of young leaves (arrowheads).