Figure 2.

ARF10 and ARF16 Control Root Cap Formation.

(A) Five-day-old seedlings showing agravitropic root growth (arf10-2 arf16-2, Pro_35S:MIR160c, and Pro_35S:MIR160c Pro_ARF17:mARF17) and rescue by expressing ARF10 or ARF16 (Pro_35S:MIR160c Pro_ARF10:mARF10, Pro_35S:MIR160c Pro_ARF16:mARF16). Pro_ARF17:mARF17 failed to rescue the gravitropism. WT, wild type. Bar = 0.5 cm.

(B) Schemes of T-DNA insertion mutants of arf10-2 and arf16-2. Black boxes and lines represent exons and introns, respectively. Triangles indicate the T-DNA insertion site.

(C) RNA gel blot of the ARF transcripts in 20-d-old wild-type (lane 1), Pro_35S:MIR160a (lane 2), Pro_35S:MIR160b (lane 3), and Pro_35S:MIR160c (lane 4) plants. In plants ectopically overexpressing miR160, most of the target transcripts were decreased and the level of cleaved products was increased. Arrows and asterisks indicate the full-length and 3′ cleaved products of each mRNA, respectively.

(D) Root tip and starch granules (stained purple) of 5-d-old seedlings.