Figure 6.

The miR160-Uncoupled Expression of mARF16 Confers Pleiotropic Effects.

(A) Root morphology of 7-d-old Pro_ARF16:ARF16 (left) and Pro_ARF16:mARF16 (right) seedlings. The Pro_ARF16:mARF16 seedlings produced fewer lateral roots. Bars = 0.5 cm.

(B) Effects of ectopic overexpression of ARF16 on the root tip. Left, Pro_35S:ARF16, which appeared normal; middle, Pro_35S:mARF16, showing the consumed basal portion of the root meristem; right, starch granules in the Pro_35S:mARF16 root tip, which appeared normal.

(C) Twenty-day-old Pro_ARF16:ARF16 (left) and Pro_ARF16:mARF16 (right) plants. Bars = 1.0 cm.

(D) Inflorescences of Pro_ARF16:ARF16 (left) and Pro_ARF16:mARF16 (right) plants. Arrows indicate the outward-curled (Pro_ARF16:ARF16) and inward-curled (Pro_ARF16:mARF16) petals. Bars = 1.0 cm.

(E) Siliques of Pro_ARF16:ARF16 (left) and Pro_ARF16:mARF16 (right) plants.

(F) Scanning electron microscopy views of adaxial epidermal cells of the first rosette leaves of 20-d-old Pro_ARF16:ARF16 (left) and Pro_ARF16:mARF16 (right) plants. Bars = 50 μm.

(G) Transverse sections of the first rosette leaves of Pro_ARF16:ARF16 (left) and Pro_ARF16:mARF16 (right) plants. The Pro_ARF16:mARF16 leaf had smaller cells, and the cell number per leaf was also reduced (data not shown). Bars = 50 μm.