Figure 7.

mARF16 Enhances Auxin Response Gene Expression in Plants.

(A) DR5-GUS expression in Pro_ARF16:ARF16 and Pro_ARF16:mARF16 plants. The ectopic expression of DR5 was most evident in the cotyledons and leaf margins when miR160 regulation was impaired. Bars = 0.5 cm.

(B) RT-PCR analysis of auxin response gene expression. The 7-d-old seedlings were treated with 50 μM IAA for 4 h in liquid half-strength MS medium. PCR was performed in 24 cycles for Actin2 (At3g18820) and 30 cycles for early auxin response genes. −, mock treatment; +, auxin treatment.