Figure 2.
Expression Patterns of WUS:GUS Constructs and Complementation of the Inflorescence Phenotype with Corresponding Genomic Fragments.
Each row shows the expression pattern of the indicated WUS promoter fragment in (from left to right) seedlings, inflorescences, and ovules and the complementation of a homozygous wus-1 mutant plant with the corresponding genomic fragment (right column). Seedlings and inflorescences were stained with GUS with 2 mM Fe-cyanide for 1 d except ScaBst (2 mM, 2 d) and Δ4 (5 mM, 1 d). Ovules were stained with GUS with 5 mM Fe-cyanide for 1 d.
(A) Truncation constructs.
(B) Deletion constructs.
b, floral bud; c, cotyledon; ch, chalaza; f, funiculus; h, hypocotyl; l, leaf; n, nucellus. Arrows indicate the shoot meristem, and the arrowhead indicates the floral meristem. Bars = 0.5 mm (seedling, inflorescence), 30 μm (ovule), 2 mm (genomic constructs, except Δ5), and 5 cm (genomic construct Δ5 and the wild type).