Figure 3.
Expression Patterns of WUS:GUS Reporter Constructs in Inflorescences.
Inflorescences ([A] to [D] and [F] to [L]) or seedling (E) were stained with either 2 mM Fe-cyanide ([A], [B], and [D] to [H]) or 5 mM Fe-cyanide ([C] and [I] to [L]) in the staining buffer for 1 d ([A] and [H] to [J]) or 3 d ([B] to [G], [K], and [L]).
(A) to (H) Whole-mount views with bright-field optics. GUS activity is visualized by blue color.
(A) Δ51.
(B) (D5)4.
(C) (D5)4 clv1-4.
(D) (G1)4.
(E) (L5)4. Staining in hydathodes (hy) and stipules (st) is attributable to background activity of the included minimal promoter.
(F) (L5)4.
(G) (S0)4.
(H) M31-HindBst (−566/−564 mutated; see Figure 4).
(I) to (L) Eight-micrometer sections viewed with dark-field optics. GUS activity is visualized by pink color.
(I) HindBst.
(J) ScaBst.
(K) (D5)4.
(L) (L5)4.
b, floral bud; fm, floral meristem; im, inflorescence meristem; s, sepal. Arrows indicate the shoot meristem, and arrowheads indicate the floral meristem. Bars = 0.5 mm ([A] and [E]), 2 mm (C), and 60 μm (I). Magnification of (B), (D), and (F) to (H) is as in (A); magnification of (J) to (L) is as in (I).