Figure 5.

Linker Scanning Analysis.

Schemes of the constructs analyzed. The 118-bp WUS promoter fragment S0 (−586/−469) was permutated with the decamer sequence ACCTCGAGTC, generating the mutated fragments S1 to S12. The −566/−557 and −546/−537 regions were also scanned with trinucleotide/tetranucleotide exchanges (M31 to M33 and M51 to M53). For the reporter constructs, each mutated fragment was tetramerized and fused to −60 CaMV:GUS. Unaltered nucleotides are indicated with dashes. Relative staining intensities in inflorescence meristems (IM) and floral meristems (FM) are indicated at right for tetrameric (tetramer) and full-length WUS promoter constructs (HindBst). All full-length promoter constructs additionally showed strong staining in ovules unaffected by the indicated mutations.