Table 1.
GC-MS Analysis of CYP85A1 and CYP85A2 Enzyme Products
| Yeast Strains | Substrates | Productsa | RRt on GCb | Prominent Ions (m/z, Relative Intensity %) |
|---|---|---|---|---|
| CYP85A1/V60/WAT21 | 6-DeoxoCS | CS | 1.000 | 512 (M+, 72), 399 (7), 358 (29), 327 (10), 287 (27), 155 (100) |
| CS | NDc | – | – | |
| [26,28-2H6]CS | ND | – | – | |
| CYP85A2/V60/WAT21 | 6-DeoxoCS | CS | 1.000 | 512 (M+, 76), 399 (8), 358 (31), 327 (7), 287 (28), 155 (100) |
| BL | 1.242 | 528 (M+, 6), 374 (39), 344 (22), 332 (40), 177 (61), 163 (26), 155 (100) | ||
| CS | BL | 1.242 | 528 (M+, 6), 374 (40), 344 (21), 332 (38), 177 (60), 163 (25), 155 (100) | |
| [26,28-2H6]CS | [26,28-2H6]BL | 1.236 | 534 (M+, 6), 374 (39), 344 (23), 338 (44), 177 (64), 163 (28), 161 (100) |
a
Analyzed by its bismethaneboronate.
b
Relative retention time (RRt) with respect to CS (29.83 min).
c
Not detected.