Table 2.

Primers and PCR conditions used to amplify targeted gene regions.

Locus	Primers	[MgCl2]	Temperature alignment	Reference
COX1	FISH-COI_F1 TCAACCAACCACAAAGACATTGGCAC	2.5 mM	56 °C	70
	FISH-COI_R1 TAGACTTCTGGGTGGCCAAAGAATCA
12S	L941-PHE AAATCAAAGCATAACACTGAAGATG	2.5 mM	48 °C	69
	H2010-VAL CCAATTTGCATGGATGTCTTCTCGG
16S	16Sa-L ACGCCTGTTTATCAAAAACAT	2.5 mM	57 °C	71
	16Sb-H CCGGTCTGAACTCAGATCACGT