FIG. 6.

Western blots for perforin and granzyme B. CD8⁺CD43⁺ T cells were purified from spleens of FV-infected mice at 2 weeks postinfection (acute) and 8 weeks postinfection (persistent), and analyzed by Western blot using anti-perforin and anti-granzyme B antibodies. Protein lysates of CD8⁺ T cells from naïve mice were used as a negative control, and immunoblotting with anti-β-actin was used to quantify the protein content in each lane. The two bands for perforin are the inactive precursor protein and the activated protein that has been proteolytically cleaved (49).