FIG. 5.

Hsp90 inhibitors alter the localization of the viral polymerase in transfected cells. Cells were transfected with a plasmid expressing the viral polymerase (UL30) and then prepared for immunofluorescence confocal microscopy as described in Materials and Methods. Cells were stained with a monoclonal antibody specific to the viral polymerase (green) and an Hsp90 polyclonal antibody (red). Merged images of untreated and treated cells are shown in panels A and B, respectively. In untreated cells, the viral polymerase localizes to the nucleus (A) as previously described (11). In cells treated with the inhibitor of Hsp90, staining for the viral polymerase is observed within the cytoplasm (B), which is consistent with its localization in infected cells treated with geldanamycin.