FIG. 4.

Cell tropism of a UL131-rapaired AD169 derivative. (A) Identification of infected HeLa and MCF-7 cells by fluorescence. Cells were infected with BADrUL131-Y4 at a multiplicity of 1 PFU/cell and fixed 48 h after infection. Nuclei were stained with DAPI (blue), and infected cells were identified by IE1 antigen staining (red) and GFP expression (green) from a marker cassette on the viruses. (B) Quantification of UL131-UL128 locus-dependent host cell tropism. Cultures of epithelial cells, endothelial cells, and fibroblasts were infected at a multiplicity of 1 PFU/cell with BADwt (black bars) or BADrUL131-Y4 (gray bars); 48 h later, cells were fixed with 2% paraformaldehyde, and infected cells were identified by monitoring GFP expression.