FIG. 1.

Kinetics of viral clearance and the LCMV-specific CD4 T-cell response. (A) Spleen and liver tissues were isolated from LCMV Armstrong-infected (ARM) or LCMV Clone 13-infected (Cl 13) animals at the indicated days postinfection, and the titers of infectious virus were determined by plaque assay. Data are expressed as PFU per gram of tissue. The dashed line represents the lower limit of detection, which is 200 PFU/gram of tissue. Each time point represents the average ± standard deviation (SD) of three mice per group. (B) The kinetics of the SMARTA T-cell response. A total of 10⁴ SMARTA cells were transferred into C57BL/6 mice that were subsequently infected with Armstrong (ARM) or Clone 13 (Cl 13). Spleen (left panel) and liver (right panel) cells were isolated at the indicated days postinfection, and the absolute number of SMARTA cells in each organ was determined. Values represent the average ± SD of three mice at each time point and are representative of at least two experiments.