TABLE 1.
Evaluation of T-20 and T-649 sensitivity of PM1-M87-selected isolates (NL/sel and BaL/sel) and pseudotyped variants carrying envelope proteins with point mutations (NL/S [L33S], BaL/V [I48V], BaL/K [N126K], BaL/VK [I48V/N126K]) in TZM-bl indicator cellsa
| Isolate | Relative fusion inhibitor resistance
|
|
|---|---|---|
| T-20 | T-649 | |
| NL4-3 | 1 | 1 |
| NL/sel | 8.0 ± 1.0 | 40.2 ± 6.3 |
| NL/S | 10.1 ± 4.3 | 50.0 ± 5.7 |
| BaL | 1 | 1 |
| BaL/sel | 16.2 ± 3.3 | 5.3 ± 1.2 |
| BaL/V | 2.4 ± 0.9 | 1.0 ± 0.4 |
| BaL/K | 5.6 ± 1.3 | 5.3 ± 0.8 |
| BaL/VK | 5.6 ± 1.5 | 2.5 ± 0.5 |
a
The mean IC50 values were calculated from at least three independent TZM-bl infections. Relative fusion inhibitor resistance was calculated by dividing the IC50 value for the PM1-M87-selected isolates and each envelope protein variant by the IC50 value for HIV-1NL4-3 or HIV-1BaL.