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. 2005 Aug;79(16):10126–10137. doi: 10.1128/JVI.79.16.10126-10137.2005

FIG. 4.

FIG. 4.

Liquid β-galactosidase assays of UPF1 null yeast transformed with plasmids encoding bicistronic mRNAs. Yeast strains AAY273 (panel A) and strain BY4741 (panel B) were transformed with different plasmids, and transformants were assayed for β-galactosidase synthesis. Labels on the x axis indicate the structure of the plasmid that was introduced into yeast strain. Clear bars, isogenic wild-type yeast strain; gray bars, mutant yeast strain. Vector: no promoter or DNA insert; promoter: ADH1 promoter only; ADE3: ADE3 gene only; lacZ: lacZ gene only; ADE3…lacZ: ADE3 and lacZ genes with no IRES; ADE3-HCV C120-lacZ: ADE3 gene, HCV IRES, amino acids 1 to 120 of the polyprotein, and lacZ; no promoter: ADE3-HCV C120-lacZ without the ADH1 promoter; G267C: ADE3-HCV C120-lacZ with a point mutation at nucleotide 267 of the HCV 5′ untranslated region. y axis, β-galactosidase activity in Miller units as determined by solution assay.