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. 2005 Aug;79(16):10126–10137. doi: 10.1128/JVI.79.16.10126-10137.2005

FIG. 7.

FIG. 7.

Liquid β-galactosidase assays of yeast deleted for genes encoding orthologs of La protein. Yeast strains CY1 (gray bars) and YSS238B (white bars) were transformed with different plasmids, and transformants were assayed for β-galactosidase synthesis. Labels on the x axis indicate the structure of the plasmid that was introduced into yeast. Vector: no promoter or DNA insert; promoter: ADH1 promoter only; ADE3: ADE3 gene only; lacZ: lacZ gene only; ADE3…lacZ: ADE3 and lacZ genes with no IRES; ADE3-HCV C5-lacZ: ADE3 gene, HCV IRES, amino acids 1 to 5 of the polyprotein, and lacZ; ADE3-HCV C120-lacZ: ADE3 gene, HCV IRES, amino acids 1 to 120 of the polyprotein, and lacZ; Δ28-69: ADE3-HCV C120-lacZ lacking nucleotides 28 to 69 of the HCV 5′ untranslated region; G267C: ADE3-HCV C120-lacZ with a point mutation at nucleotide 267 of the HCV 5′ untranslated region. y axis, β-galactosidase activity in Miller units as determined by solution assay.