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GFP expression levels. Vero cells were infected at an MOI of 1 with the NS1-GFPStSt (a) or NS1-GFP (b) virus, in which GFP is fused to NS1 and posttranslationally cleaved. At 24 h postinfection, fluorescence was analyzed using confocal microscopy (a and b) or FACS (c).